primers of cycline Search Results


cyclin d1 hs00765553 primers  (Thermo Fisher)
93
Thermo Fisher cyclin d1 hs00765553 primers
Cyclin D1 Hs00765553 Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primers for cyclin d1 proximal promoter  (Biotechnology Information)
90
Biotechnology Information primers for cyclin d1 proximal promoter
Primers For Cyclin D1 Proximal Promoter, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
primers for cyclin d1 proximal promoter - by Bioz Stars, 2026-04
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primer pairs cycline d1  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation primer pairs cycline d1
Primer Pairs Cycline D1, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cyclin a2 primers  (Qiagen)
90
Qiagen mouse cyclin a2 primers
Mouse Cyclin A2 Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cyclin a2 primers - by Bioz Stars, 2026-04
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cyclin b1 n upper primers  (Fisher Scientific)
90
Fisher Scientific cyclin b1 n upper primers
Cyclin B1 N Upper Primers, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cyclin b1 n upper primers - by Bioz Stars, 2026-04
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primers for human cyclin d1  (Qiagen)
90
Qiagen primers for human cyclin d1
Primers For Human Cyclin D1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers for human cyclin d1/product/Qiagen
Average 90 stars, based on 1 article reviews
primers for human cyclin d1 - by Bioz Stars, 2026-04
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quantitect primer assays for cyclin b1  (Qiagen)
90
Qiagen quantitect primer assays for cyclin b1
Regulation of the cell cycle checkpoints. a Relative mRNA expression of the cell cycle regulators <t>cyclin</t> <t>B1,</t> CDK1, and CDK2 after 48 h incubation with 30 and 100 μM diacerein. Asterisks represent significant differences between control and treated cells (* p < 0.05; ** p < 0.01; *** p < 0.001). b Total protein analysis after 48 h of treatment revealed a significant down-regulation of the G2/M arrest regulator proteins cyclin B1, CDK1, and CDK2 in 100 and 300 μM diacerein treated chondrosarcoma cells. β-actin was used as loading control. c Quantitative evaluation of the western blot analysis of cell cycle regulator proteins
Quantitect Primer Assays For Cyclin B1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantitect primer assays for cyclin b1/product/Qiagen
Average 90 stars, based on 1 article reviews
quantitect primer assays for cyclin b1 - by Bioz Stars, 2026-04
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primer sets for rat genes plk1, cdc25b, bub1b, and cyclin f  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation primer sets for rat genes plk1, cdc25b, bub1b, and cyclin f
Regulation of the cell cycle checkpoints. a Relative mRNA expression of the cell cycle regulators <t>cyclin</t> <t>B1,</t> CDK1, and CDK2 after 48 h incubation with 30 and 100 μM diacerein. Asterisks represent significant differences between control and treated cells (* p < 0.05; ** p < 0.01; *** p < 0.001). b Total protein analysis after 48 h of treatment revealed a significant down-regulation of the G2/M arrest regulator proteins cyclin B1, CDK1, and CDK2 in 100 and 300 μM diacerein treated chondrosarcoma cells. β-actin was used as loading control. c Quantitative evaluation of the western blot analysis of cell cycle regulator proteins
Primer Sets For Rat Genes Plk1, Cdc25b, Bub1b, And Cyclin F, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer sets for rat genes plk1, cdc25b, bub1b, and cyclin f/product/SuperArray Bioscience Corporation
Average 90 stars, based on 1 article reviews
primer sets for rat genes plk1, cdc25b, bub1b, and cyclin f - by Bioz Stars, 2026-04
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mrna primers for cyclin d1  (Genechem)
90
Genechem mrna primers for cyclin d1
Regulation of the cell cycle checkpoints. a Relative mRNA expression of the cell cycle regulators <t>cyclin</t> <t>B1,</t> CDK1, and CDK2 after 48 h incubation with 30 and 100 μM diacerein. Asterisks represent significant differences between control and treated cells (* p < 0.05; ** p < 0.01; *** p < 0.001). b Total protein analysis after 48 h of treatment revealed a significant down-regulation of the G2/M arrest regulator proteins cyclin B1, CDK1, and CDK2 in 100 and 300 μM diacerein treated chondrosarcoma cells. β-actin was used as loading control. c Quantitative evaluation of the western blot analysis of cell cycle regulator proteins
Mrna Primers For Cyclin D1, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrna primers for cyclin d1 - by Bioz Stars, 2026-04
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ccnb2 antibodies d260235  (Sangon Biotech)
90
Sangon Biotech ccnb2 antibodies d260235
The expression levels of CDK1, CDK5, CDC20, CCNA2, CCNB1 and <t>CCNB2</t> in HCC. (A) Transcriptional pattern of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in major malignant tumors compared with those in normal tissues using the Oncomine database. The heat-map represents data with statistically significant upregulation (red) or downregulation (blue). The numbers in the heat-map indicate the published independent datasets of mRNA microarray experiments. (B) The protein expression levels of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HCC from HPA. Scale bar: 200μm. (C) The mRNA expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in GEPIA. (* P < 0.05). (D) The mRNA expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 compared with the normal tissues in HCCDB. (E) Correlation of the CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 expression levels with various tumor stages among HCC tissues in GEPIA.
Ccnb2 Antibodies D260235, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primers for cdc25c  (Qiagen)
90
Qiagen primers for cdc25c
Defective type II cell proliferation in mouse lungs having FoxM1 deleted in type II cells. (A and B) Double labeling of BrdU and Sp-C in WT (A) and mutant (Mut; SPC-rtTA/TetO-Cre/FOXM1EKO ; B) lungs at 72 h after infection. Small blood vessels are indicated by thin white lines (B) or the circled area (A). (C and D) Higher magnification images of the blue rectangles in A and B, respectively. Arrow shows BrdU colocalization with Sp-C in a WT cell. Data are representative of three independent experiments. (E) Percentage of BrdU + Sp-C + cells among Sp-C + cells in WT and mutant at 72 h after infection. (F) Type II cells were isolated from ROSA-YFP-EKO mice. YFP + and YFP − cells were separated and labeled with BrdU for 24 h. The percentage of BrdU + cells among YFP + (mutant) or YFP − (WT) type II cells were compared. (E and F) Data are presented as mean ± SE (*, P < 0.05 vs. control). 100–500 type II cells were scored in each sample ( n = 3 mice for each group from three independent experiments). (G and H) Relative expression of <t>CDC25C</t> (G) and CyclinB1 (H) in uninfected WT cells (basal) and in WT (Con) and FoxM1 −/− type II cells at 72 h after PA infection as assessed by real-time RT-PCR. Data are presented as mean ± SE ( n = 3–4 mice for each group from three independent experiments; *, P < 0.05 vs. control). (I and J) Isolated WT, non–PA-treated type II cells cultured with BrdU were infected with control adenovirus (Con adv; I) or adenovirus expressing FoxM1 (FoxM1 adv; J). Data are representative of three independent experiments. Bars: (A and B) 60 µm; (C and D) 25 µm; (I and J) 20 µm.
Primers For Cdc25c, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primers for cyclin d1 p298560  (Bioneer Corporation)
90
Bioneer Corporation primers for cyclin d1 p298560
Defective type II cell proliferation in mouse lungs having FoxM1 deleted in type II cells. (A and B) Double labeling of BrdU and Sp-C in WT (A) and mutant (Mut; SPC-rtTA/TetO-Cre/FOXM1EKO ; B) lungs at 72 h after infection. Small blood vessels are indicated by thin white lines (B) or the circled area (A). (C and D) Higher magnification images of the blue rectangles in A and B, respectively. Arrow shows BrdU colocalization with Sp-C in a WT cell. Data are representative of three independent experiments. (E) Percentage of BrdU + Sp-C + cells among Sp-C + cells in WT and mutant at 72 h after infection. (F) Type II cells were isolated from ROSA-YFP-EKO mice. YFP + and YFP − cells were separated and labeled with BrdU for 24 h. The percentage of BrdU + cells among YFP + (mutant) or YFP − (WT) type II cells were compared. (E and F) Data are presented as mean ± SE (*, P < 0.05 vs. control). 100–500 type II cells were scored in each sample ( n = 3 mice for each group from three independent experiments). (G and H) Relative expression of <t>CDC25C</t> (G) and CyclinB1 (H) in uninfected WT cells (basal) and in WT (Con) and FoxM1 −/− type II cells at 72 h after PA infection as assessed by real-time RT-PCR. Data are presented as mean ± SE ( n = 3–4 mice for each group from three independent experiments; *, P < 0.05 vs. control). (I and J) Isolated WT, non–PA-treated type II cells cultured with BrdU were infected with control adenovirus (Con adv; I) or adenovirus expressing FoxM1 (FoxM1 adv; J). Data are representative of three independent experiments. Bars: (A and B) 60 µm; (C and D) 25 µm; (I and J) 20 µm.
Primers For Cyclin D1 P298560, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primers for cyclin d1 p298560/product/Bioneer Corporation
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Image Search Results


Regulation of the cell cycle checkpoints. a Relative mRNA expression of the cell cycle regulators cyclin B1, CDK1, and CDK2 after 48 h incubation with 30 and 100 μM diacerein. Asterisks represent significant differences between control and treated cells (* p < 0.05; ** p < 0.01; *** p < 0.001). b Total protein analysis after 48 h of treatment revealed a significant down-regulation of the G2/M arrest regulator proteins cyclin B1, CDK1, and CDK2 in 100 and 300 μM diacerein treated chondrosarcoma cells. β-actin was used as loading control. c Quantitative evaluation of the western blot analysis of cell cycle regulator proteins

Journal: BMC Cancer

Article Title: Diacerein retards cell growth of chondrosarcoma cells at the G2/M cell cycle checkpoint via cyclin B1/CDK1 and CDK2 downregulation

doi: 10.1186/s12885-015-1915-4

Figure Lengend Snippet: Regulation of the cell cycle checkpoints. a Relative mRNA expression of the cell cycle regulators cyclin B1, CDK1, and CDK2 after 48 h incubation with 30 and 100 μM diacerein. Asterisks represent significant differences between control and treated cells (* p < 0.05; ** p < 0.01; *** p < 0.001). b Total protein analysis after 48 h of treatment revealed a significant down-regulation of the G2/M arrest regulator proteins cyclin B1, CDK1, and CDK2 in 100 and 300 μM diacerein treated chondrosarcoma cells. β-actin was used as loading control. c Quantitative evaluation of the western blot analysis of cell cycle regulator proteins

Article Snippet: The following primers were used for real time RT-PCR: QuantiTect primer assays (Qiagen) for cyclin B1 (ID QT00006615), CDK1 (ID QT00042672), and CDK2 (ID QT00005586).

Techniques: Expressing, Incubation, Control, Western Blot

The expression levels of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HCC. (A) Transcriptional pattern of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in major malignant tumors compared with those in normal tissues using the Oncomine database. The heat-map represents data with statistically significant upregulation (red) or downregulation (blue). The numbers in the heat-map indicate the published independent datasets of mRNA microarray experiments. (B) The protein expression levels of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HCC from HPA. Scale bar: 200μm. (C) The mRNA expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in GEPIA. (* P < 0.05). (D) The mRNA expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 compared with the normal tissues in HCCDB. (E) Correlation of the CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 expression levels with various tumor stages among HCC tissues in GEPIA.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: The expression levels of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HCC. (A) Transcriptional pattern of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in major malignant tumors compared with those in normal tissues using the Oncomine database. The heat-map represents data with statistically significant upregulation (red) or downregulation (blue). The numbers in the heat-map indicate the published independent datasets of mRNA microarray experiments. (B) The protein expression levels of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HCC from HPA. Scale bar: 200μm. (C) The mRNA expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in GEPIA. (* P < 0.05). (D) The mRNA expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 compared with the normal tissues in HCCDB. (E) Correlation of the CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 expression levels with various tumor stages among HCC tissues in GEPIA.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing, Microarray

(A) The expression of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in HepG2 and BEL-7402 cells compared with LO2 cells by quantitative PCR analysis. *p <0.05, **p <0.01, ***p <0.001; n = 3. (B) The subcellular localization of the six-cell cycle related protein analyzed in HPA database. (C) Immunofluorescence images of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HepG2 cells. The nucleuses were stained blue, the microtubules were stained red, and the cell cycle related proteins were stained green. Scale bar = 10μm.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: (A) The expression of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in HepG2 and BEL-7402 cells compared with LO2 cells by quantitative PCR analysis. *p <0.05, **p <0.01, ***p <0.001; n = 3. (B) The subcellular localization of the six-cell cycle related protein analyzed in HPA database. (C) Immunofluorescence images of CDK1, CDK5, CDC20, CCNA2, CCNB1 and CCNB2 in HepG2 cells. The nucleuses were stained blue, the microtubules were stained red, and the cell cycle related proteins were stained green. Scale bar = 10μm.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

Expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in HCC in the presence of TP53 mutation status in UALCAN (A) and (B) GEPIA database.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: Expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 in HCC in the presence of TP53 mutation status in UALCAN (A) and (B) GEPIA database.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing, Mutagenesis

Contributions of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 mRNA expression levels in predicting prognosis of HCC patients (Kaplan-Meier plotter). (A) Relationship between high expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 with poor OS for HCC patients (n = 364). (B) Relationship between high expression levels of CDK1 , CDC20 , CCNA2 , CCNB1 and CCNB2 with poor PFS for HCC patients (n = 370). OS, overall survival; PFS, Relapse free survival.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: Contributions of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 mRNA expression levels in predicting prognosis of HCC patients (Kaplan-Meier plotter). (A) Relationship between high expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 with poor OS for HCC patients (n = 364). (B) Relationship between high expression levels of CDK1 , CDC20 , CCNA2 , CCNB1 and CCNB2 with poor PFS for HCC patients (n = 370). OS, overall survival; PFS, Relapse free survival.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing

The relationship between the expression of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 , CCNB2 and the level of HCC immune infiltration. The expressions of CDK1 , CDC20 , CCNA2 , CCNB1 and CCNB2 were significantly positively correlated with the infiltration levels of B cells, CD8+ T cells, CD4+ T cells, neutrophils, macrophages and DCs in HCC (n = 457). CDK5 was positively correlated with infiltrating level of B cell, Macrophage, neutrophils and DCs.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: The relationship between the expression of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 , CCNB2 and the level of HCC immune infiltration. The expressions of CDK1 , CDC20 , CCNA2 , CCNB1 and CCNB2 were significantly positively correlated with the infiltration levels of B cells, CD8+ T cells, CD4+ T cells, neutrophils, macrophages and DCs in HCC (n = 457). CDK5 was positively correlated with infiltrating level of B cell, Macrophage, neutrophils and DCs.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing

Correlation Analysis between Expression of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 and Levels of Markers of Immune Cells in TIMER

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: Correlation Analysis between Expression of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 and Levels of Markers of Immune Cells in TIMER

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing

The expression levels of CDK1 , CDC20 , CCNA2 , CCNB1 and CCNB2 were correlated with the polarization of macrophages in HCC. Markers included CSF1R and CD86 of monocytes; IL10 and CD68 of TAMs (tumor-associated macrophages). ( A ) Scatterplots of correlations between six cell cycle-related genes expression and gene markers of monocytes. ( B ) Scatterplots of correlations between six cell cycle-related genes expression and gene markers of TAMs.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: The expression levels of CDK1 , CDC20 , CCNA2 , CCNB1 and CCNB2 were correlated with the polarization of macrophages in HCC. Markers included CSF1R and CD86 of monocytes; IL10 and CD68 of TAMs (tumor-associated macrophages). ( A ) Scatterplots of correlations between six cell cycle-related genes expression and gene markers of monocytes. ( B ) Scatterplots of correlations between six cell cycle-related genes expression and gene markers of TAMs.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing

Gene co-expression among HCC cases. (A) Gene co-expression among HCC cases (STRING). (B) Functions of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 showed positive correlation with these genes alterations. (C) Network of KEGG and GO enriched terms colored by clusters. (D) Network of KEGG and GO enriched terms colored by P -value (Metascape database).

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: Gene co-expression among HCC cases. (A) Gene co-expression among HCC cases (STRING). (B) Functions of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 showed positive correlation with these genes alterations. (C) Network of KEGG and GO enriched terms colored by clusters. (D) Network of KEGG and GO enriched terms colored by P -value (Metascape database).

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing

The relationship between the expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 and the prognosis of HCC with risk factors. (A) Relationship of up-regulation expression levels of six cell cycle-related genes with OS (n = 117) and PFS (n = 169) of HCC patients with AC, (B) The correlation between high transcription levels of six cell cycle-related genes with OS (n = 153) and PFS (n = 205) of HCC patients with HV.

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: The relationship between the expression levels of CDK1 , CDK5 , CDC20 , CCNA2 , CCNB1 and CCNB2 and the prognosis of HCC with risk factors. (A) Relationship of up-regulation expression levels of six cell cycle-related genes with OS (n = 117) and PFS (n = 169) of HCC patients with AC, (B) The correlation between high transcription levels of six cell cycle-related genes with OS (n = 153) and PFS (n = 205) of HCC patients with HV.

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques: Expressing

P -value of the six up-regulated cell cycle-related genes in HCCDB database

Journal: Journal of Cancer

Article Title: Six Cell Cycle-related Genes Serve as Potential Prognostic Biomarkers and Correlated with Immune Infiltrates in Hepatocellular Carcinoma

doi: 10.7150/jca.76809

Figure Lengend Snippet: P -value of the six up-regulated cell cycle-related genes in HCCDB database

Article Snippet: Cells were blocked with 5% BSA, and incubated with CDK1 antibodies (Sangon Biotech, D190678), CDK5 antibodies (Sangon Biotech, D199878), CDC20 antibodies (Sangon Biotech, D120392), CCNA2 antibodies (Sangon Biotech, D160233), CCNB1 antibodies (Sangon Biotech, D260234), and CCNB2 antibodies (Sangon Biotech, D260235), at 4 °C overnight.

Techniques:

Defective type II cell proliferation in mouse lungs having FoxM1 deleted in type II cells. (A and B) Double labeling of BrdU and Sp-C in WT (A) and mutant (Mut; SPC-rtTA/TetO-Cre/FOXM1EKO ; B) lungs at 72 h after infection. Small blood vessels are indicated by thin white lines (B) or the circled area (A). (C and D) Higher magnification images of the blue rectangles in A and B, respectively. Arrow shows BrdU colocalization with Sp-C in a WT cell. Data are representative of three independent experiments. (E) Percentage of BrdU + Sp-C + cells among Sp-C + cells in WT and mutant at 72 h after infection. (F) Type II cells were isolated from ROSA-YFP-EKO mice. YFP + and YFP − cells were separated and labeled with BrdU for 24 h. The percentage of BrdU + cells among YFP + (mutant) or YFP − (WT) type II cells were compared. (E and F) Data are presented as mean ± SE (*, P < 0.05 vs. control). 100–500 type II cells were scored in each sample ( n = 3 mice for each group from three independent experiments). (G and H) Relative expression of CDC25C (G) and CyclinB1 (H) in uninfected WT cells (basal) and in WT (Con) and FoxM1 −/− type II cells at 72 h after PA infection as assessed by real-time RT-PCR. Data are presented as mean ± SE ( n = 3–4 mice for each group from three independent experiments; *, P < 0.05 vs. control). (I and J) Isolated WT, non–PA-treated type II cells cultured with BrdU were infected with control adenovirus (Con adv; I) or adenovirus expressing FoxM1 (FoxM1 adv; J). Data are representative of three independent experiments. Bars: (A and B) 60 µm; (C and D) 25 µm; (I and J) 20 µm.

Journal: The Journal of Experimental Medicine

Article Title: FoxM1 mediates the progenitor function of type II epithelial cells in repairing alveolar injury induced by Pseudomonas aeruginosa

doi: 10.1084/jem.20102041

Figure Lengend Snippet: Defective type II cell proliferation in mouse lungs having FoxM1 deleted in type II cells. (A and B) Double labeling of BrdU and Sp-C in WT (A) and mutant (Mut; SPC-rtTA/TetO-Cre/FOXM1EKO ; B) lungs at 72 h after infection. Small blood vessels are indicated by thin white lines (B) or the circled area (A). (C and D) Higher magnification images of the blue rectangles in A and B, respectively. Arrow shows BrdU colocalization with Sp-C in a WT cell. Data are representative of three independent experiments. (E) Percentage of BrdU + Sp-C + cells among Sp-C + cells in WT and mutant at 72 h after infection. (F) Type II cells were isolated from ROSA-YFP-EKO mice. YFP + and YFP − cells were separated and labeled with BrdU for 24 h. The percentage of BrdU + cells among YFP + (mutant) or YFP − (WT) type II cells were compared. (E and F) Data are presented as mean ± SE (*, P < 0.05 vs. control). 100–500 type II cells were scored in each sample ( n = 3 mice for each group from three independent experiments). (G and H) Relative expression of CDC25C (G) and CyclinB1 (H) in uninfected WT cells (basal) and in WT (Con) and FoxM1 −/− type II cells at 72 h after PA infection as assessed by real-time RT-PCR. Data are presented as mean ± SE ( n = 3–4 mice for each group from three independent experiments; *, P < 0.05 vs. control). (I and J) Isolated WT, non–PA-treated type II cells cultured with BrdU were infected with control adenovirus (Con adv; I) or adenovirus expressing FoxM1 (FoxM1 adv; J). Data are representative of three independent experiments. Bars: (A and B) 60 µm; (C and D) 25 µm; (I and J) 20 µm.

Article Snippet: Primers are listed in Table S1 ; the primers for CDC25C, Cyclin B1, and β-catenin were obtained from QIAGEN.

Techniques: Labeling, Mutagenesis, Infection, Isolation, Control, Expressing, Quantitative RT-PCR, Cell Culture